Developmental Competence of Intrafollicular Oocytes Derived from Preantral Follicle Culture with Different Protocols after Parthenogenetic Activation

P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...

The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles

P-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes

Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...

Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence.

BACKGROUND We investigated at what stage early cultured preantral mouse follicles become dependent on a minimal effective FSH dose (10 mIU/ml) and analysed the influence of implementing FSH at several time-points during in vitro culture. METHODS Two-layered mouse follicles were cultured for 12 days under seven different FSH exposure regimens and ovulated on day 13 by hCG/EGF. Ovulated cumulus...

Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts

These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-like (ESCs) derived from porcine parthenogenetic blastocysts (pPBs). These results showed that, as the electric field strength increased from 1.0 to 2.7 kV/cm, the cleavage rate of parthenogenetic ...